Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals

doi: 10.1186/s12958-018-0377-3

Figure Lengend Snippet: Primer sequences of PCR

Article Snippet: Antibodies against β-actin (1:5000, Proteintech, IL, USA), IGFBP1 (1:1000, Proteintech, IL, USA), ITGB3 (1:1000, Proteintech, IL, USA), MUC1 (1:1000, Proteintech, IL, USA), ACVR1B (1:1000, Proteintech, IL, USA) and STAT3 (1:1000, Cell Signaling Technology, Danvers, MA, USA) were used as primary antibodies, and horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5000, Cell Signaling Technology, Danvers, MA, USA) and goat anti-mouse IgG (1:5000, Cell Signaling Technology, Danvers, MA, USA) were used as secondary antibodies.

Techniques:

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Effects of Levonorgestrel and progesterone on Oviductal physiology in mammals

doi: 10.1186/s12958-018-0377-3

Figure Lengend Snippet: Effects of different concentrations of LNG and P4 on the expression of receptivity markers in the fallopian tubes. a-b mRNA expression levels of LIF, STAT3, IGFBP1, ITGB3, MUC1, and ACVR1B in OE-E6/E7 cells following treatment with different concentrations of LNG and P4 ( n = 3 in each group; ns, not significant); c protein expression levels of MUC1, ITGB3, ACVR1B, STAT3, and IGFBP1 in OE-E6/E7 cells following treatment with different concentrations of LNG and P4

Article Snippet: Antibodies against β-actin (1:5000, Proteintech, IL, USA), IGFBP1 (1:1000, Proteintech, IL, USA), ITGB3 (1:1000, Proteintech, IL, USA), MUC1 (1:1000, Proteintech, IL, USA), ACVR1B (1:1000, Proteintech, IL, USA) and STAT3 (1:1000, Cell Signaling Technology, Danvers, MA, USA) were used as primary antibodies, and horseradish peroxidase-conjugated goat anti-rabbit IgG (1:5000, Cell Signaling Technology, Danvers, MA, USA) and goat anti-mouse IgG (1:5000, Cell Signaling Technology, Danvers, MA, USA) were used as secondary antibodies.

Techniques: Expressing